Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

Background and Aim: Staphylococcus aureus pseudintermedius are widespread skin mucous membrane colonizers may cause opportunistic infections in humans animals. This study aimed to identify characterize methicillin-resistant S. (MRSA) (MRSP) isolates from domestic stray dogs cats pet owners Malaysia using molecular epidemiology antimicrobial profiling. Materials Methods: Three hundred fifty oral nasal swabs were taken owners; all samples subjected culture biochemical tests polymerase chain reaction; the selected put through disk diffusion test multilocus sequence typing. Results: One isolate three identified as MRSA MRSP, respectively, of which one MRSP showed multidrug resistance remaining two resistant or antimicrobials. Multilocus typing that belongs clonal complex (CC) 789, while for isolates, CC45 was a singleton. Conclusion: is first perform characterization isolated owners. The outcomes this reveal even healthy their can be carriers drug-resistant staphylococci, highlighting role pets Malaysia. Keywords: resistance, susceptibility testing, methicillin typing, public health, zoonotic infections.